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antibrca1  (R&D Systems)


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    Structured Review

    R&D Systems antibrca1
    Antibrca1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibrca1/Human+BRCA1+N-Terminus+Antibody/pm38565883-222-49-53
    Average 92 stars, based on 2 article reviews
    antibrca1 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Incubation:

    Article Title: Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade.
    Article Snippet: Proteins were separated by SDS-PAGE gel and transferred to a PVDF membrane (Millipore, IPVH00010). .. The membranes were blocked with 5% non-fat milk and incubated with primary antibodies overnight at 4 °C with gentle agitation using the following antibodies and dilutions: anti-β-actin (1:1000; Cell Signaling, Cat#: 4970, RRID:AB_2223172), anti-BRCA2 (1:500; Cell Signaling, Cat#: 10741, RRID:AB_2797730), anti-BRCA1 (cterminus, 1:200; Santa Cruz, Cat#: sc-6954, RRID: AB_626761), antiBRCA1 (n-terminus, 1 μg/mL, R&D Systems, Cat#: AF2210, RRID:AB_2067618), anti-MGAT5 for mouse (1:500; Thermo Fisher, Cat#: PA5-87988, RRID:AB_2804566), anti-MGAT5 for human (1:500; R&D Systems, Cat#: MAB5469, RRID:AB_10972310). .. Objective signals were amplified with HRP-conjugated secondary antibodies (1:2000; Cell Signaling, Cat#: 7076, RRID:AB_330924; 1:3000; Cat#: 7074, RRID:AB_2099233; and 1:5000; Thermo Fisher, Cat#: 31402, RRID:AB_228395) and detected by chemiluminescent substrate (Thermo Fisher, Cat#: 34094).

    Gentle:

    Article Title: Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade.
    Article Snippet: Proteins were separated by SDS-PAGE gel and transferred to a PVDF membrane (Millipore, IPVH00010). .. The membranes were blocked with 5% non-fat milk and incubated with primary antibodies overnight at 4 °C with gentle agitation using the following antibodies and dilutions: anti-β-actin (1:1000; Cell Signaling, Cat#: 4970, RRID:AB_2223172), anti-BRCA2 (1:500; Cell Signaling, Cat#: 10741, RRID:AB_2797730), anti-BRCA1 (cterminus, 1:200; Santa Cruz, Cat#: sc-6954, RRID: AB_626761), antiBRCA1 (n-terminus, 1 μg/mL, R&D Systems, Cat#: AF2210, RRID:AB_2067618), anti-MGAT5 for mouse (1:500; Thermo Fisher, Cat#: PA5-87988, RRID:AB_2804566), anti-MGAT5 for human (1:500; R&D Systems, Cat#: MAB5469, RRID:AB_10972310). .. Objective signals were amplified with HRP-conjugated secondary antibodies (1:2000; Cell Signaling, Cat#: 7076, RRID:AB_330924; 1:3000; Cat#: 7074, RRID:AB_2099233; and 1:5000; Thermo Fisher, Cat#: 31402, RRID:AB_228395) and detected by chemiluminescent substrate (Thermo Fisher, Cat#: 34094).



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